INTER AND INTRA-SPECIFIC VARIATION OF THE SHELL OF TWO SPECIES OF THE TROPICAL FRESHWATER SNAIL BELLAMYA (MOLLUSCA: PROSOBRANCHIA)

Shell variation in two species of the tropical freshwater snailBellamya from two different habitats, was studied. The variation of the shell features studied, which includedthe shell width, spire height, aperture height and aperturewidth, were found to be related to shell height. Furthermore,significant difference in the shell shape was found not onlybetween the two species but also between males and females ofeach species. (Received 18 August 1983;     

In vitro Regeneration of Mustard Plants (Brassica juncea var. RAI-5) on Cotyledon Explants from Non-irradiated, Irradiated and Mutagen-treated seed

Shoot formation in cotyledon explants of mustard (Brassica junceavar. Rai-5) was observed on Murashige and Skoog's medium supplementedwith NAA^* (1 mg l–1) and BA (1 mg l–1). Hypocotylsegments failed to differentiate shoots. Complete plants wereobtained when shoots were rooted in MS medium with NAA (1 mgl–1). EMS, a chemical mutagen, had an inhibitory effecton shoot regeneration. Gamma rays in doses above 2 kR suppressedshoot regeneration but stimulated callus growth. Brassica juncea, mustard, regeneration, tissue culture     

Control of the haematophagous fly Hippobosca maculata, a serious pest of equines, by deltamethrin

Abstract. This study reports the results of both a small-scale and a large-scale field treatment to assess control of the haematophagous fly Hippobosca maculata Leach a serious pest of equines in a stud in India using a deltamethrin based formulation, Butox^®. In the small-scale field trial application of 2 litres of deltamethrin at 0.001-0.003% concentration gave 90–100% control over 30 days. At 0.004% and 0.005% concentrations complete control was recorded for 45 and 90 days respectively. Mass application of 2 litres of 0.005% deltamethrin to equines and bovines controlled infestations of H.maculata for 1 year.     

体外重构STUB1介导α-1抗胰蛋白酶突变体Z蛋白泛素化修饰反应体系

α-1抗胰蛋白酶Z型突变体蛋白(α-1 antitrypsin Z-mutant protein, ATZ)是引发α-1抗胰蛋白酶缺陷症(α-1 antitrypsin deficiency, AATD)的主要原因,研究ATZ蛋白的泛素化修饰和降解对于治疗AATD具有重要意义。STUB1是一种重要的E3泛素连接酶,参与调节多种蛋白质的泛素化修饰。然而,STUB1是否参与ATZ的泛素化修饰尚未明确。本研究首先将ATZ和STUB1的编码基因克隆到pET28a质粒,构建了这2个蛋白的表达质粒。随后,将重组质粒转入大肠杆菌表达系统,在优化诱导条件实现了重组蛋白的异源表达。通过金属螯合亲和层析技术纯化得到目的蛋白,并通过蛋白质谱分析验证了其氨基酸序列的准确性。利用纯化的ATZ和STUB1重组蛋白,构建了一个体外泛素化修饰反应体系。实验结果显示,在ATP、E1泛素激活酶和E2泛素结合酶的协同作用下,STUB1成功催化了ATZ的泛素化修饰。本研究提供了一种体外获得Z型突变体ATZ纯化蛋白的方法,并确认了STUB1介导ATZ的泛素化修饰功能,推进了对α-1抗胰蛋白酶Z型突变体蛋白在细胞内降解过程的调控机制的理解。     

高黎贡山雉类生境预测

物种分布信息对野生动物的保护和管理至关重要。基于物种访查数据和气候数据,采用基于物种生境偏好、利比希最小因子定律和谢尔福德耐受性定律构建的生态位模型,综合考虑物种出现点和环境变量,预测了高黎贡山白尾梢虹雉Lophophorus sclateri、血雉Ithaginis cruentus、白鹇Lophura nycthemera、白腹锦鸡Chrysolophus amherstiae的潜在分布区域。结果表明,模型对4种雉类的预测均达到较好效果;白尾梢虹雉、血雉、白鹇和白腹锦鸡的潜在生境总面积分别为6 432 km^2、8 464 km^2、9 573 km^2和13 691 km^2,白鹇和白腹锦鸡的潜在生境面积大于白尾梢虹雉和血雉,但后两者具有更多的高质量生境。高黎贡山北段是4种雉类潜在生境的重叠区,为高黎贡山雉类保护的优先区域,尤其是白尾梢虹雉,建议加大该区域的雉类调查和保护力度。     

HPV感染结合负荷量检测对宫颈癌及上皮内瘤变的诊断价值

目的探讨HPV感染(尤其是高危型HPV)及其病毒负荷量在宫颈癌及上皮内瘤变诊断中的价值,以期对临床工作有所借鉴。方法采取回顾性研究宫颈癌、上皮内瘤变及正常妇女387例,对比分析宫颈癌、上皮内瘤变I、II、III级及正常妇女的HPV感染率及负荷情况。结果 (1)CIN I、II、III级和宫颈癌HPV感染率明显高于正常组(70.45%,55.56%,66.33%,84.62%vs 13.19%,P0.05),以高危型HPV为主(各占47.73%,41.67%,59.18%,83.08%),并且单一感染多见(63.64%,47.22%,53.06%,67.69%):(2)CIN I级HPV-DNA负荷量较低,CIN II、III级和宫颈癌组负荷量逐渐增加,尤其是病毒负荷量达500~1000及以上的比例逐步增高(P0.05):(3)Pearson相关分析可见HPV负荷量与宫颈病变严重程度密切相关(P0.01)。结论宫颈癌和CIN多伴有高危型HPV感染,其病变严重程度与HPV负荷量密切相关,HPV结合负荷量检测能更精确判断宫颈病变。